Composite

Part:BBa_K3505025:Design

Designed by: Asteria Tsapadikou,Emmanouil Stylianakis   Group: iGEM20_Thessaly   (2020-10-20)


ParaBAD:RBS-FFAR2:AVPR2 tail:TCS-LacI-terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1527
    Illegal PstI site found at 2107
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1527
    Illegal PstI site found at 2107
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1148
    Illegal BamHI site found at 1349
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1527
    Illegal PstI site found at 2107
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1527
    Illegal PstI site found at 2107
    Illegal NgoMIV site found at 1607
    Illegal AgeI site found at 983
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 965
    Illegal SapI site found at 1803
    Illegal SapI site found at 2058
    Illegal SapI.rc site found at 1260


Design Notes

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Source

source

References